Mass-produced Spirulina-mediated altered responses in ARPE-19 and HaCaT cells for biomedical applications

초록

Spirulina has been highlighted for potential health benefits based on its therapeutic functions. In the present study, the physicochemical characteristics and effects of Spirulina on ARPE-19 and HaCaT cells were monitored after mass-production, compared with conventional product. Gene ontology (GO) was analyzed using systems biology network that accounted for the function of Spirulina in human health. Spirulina powder had a smooth surface with adsorbing particles and contained sodium, magnesium, and potassium. Spirulina powder extracts in 0.1 x PBS, 0.1 M HEPES, 0.1 M NaCl, ethanol, isopropyl alcohol, and acetonitrile showed broad absorbance peaks at 300-450 and 500-700 nm. In ARPE-19 and HaCaT cells, Spirulina powder in PBS displayed proliferation at 2-20 mg/mL and 2 mg/mL, respectively. Cell viability levels of HaCaT cells were almost higher than 90% at 0.1-20 mg/mL. Despite cell susceptibility, phycocyanin, which is a component of Spirulina, helped maintain consistency of cell responses with Spirulina. Spirulina functions were explained by GO-terms, mainly binding, cellular process, and cell in the network, in terms of anti-oxidant activity. Mass-produced Spirulina powder can be developed as a high-value nutraceutical for therapeutic applications in clinics. This protocol can be extended to other materials for biomedical applications. (c) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

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