SB365 Isolated from Pulsatilla koreana Induce Apoptosis in Human Brain Glioblastoma Cells

초록

Background: Development of small molecules that inhibit cancer progression is especially important in brain tumor by potentially being able to cross the blood brain barrier. SB365, Pulsatilla saponin D from the root of Pulsatilla koreana Pulsatil has known to inhibit proliferation and induce apoptosis in hepatocellular carcinoma and colon cancer cell lines. This study was planned to determine the effect that SB365, has on apoptosis in human glioblastoma cell lines. Methods: We examined the effect of SB365 on human glioblastoma cell lines: U87MG, A172, and T98G. Apoptosis was measured by evaluating terminal deoxynucleotidyl transferase dUTP nick end labeling assay and DAPI nuclear staining. The expression of cleaved poly(ADP-ribose) polymerase (PARP), Bcl-2, Bax, and cleaved caspase-3 were assayed by western blot analysis in cells treated with SB365. Results: High rates of apoptosis were induced by SB365 in the glioblastoma cell lines. The proliferative rates of brain giloblastoma cells were definitely suppressed by SB365 in a dose dependent and time dependent manner. The outcome of Western blotting showed that protein expressions of cleaved PARP, bax and cleaved caspase-3 were increased, however Bcl-2 was markedly decreased. SB365 demonstrated potent anti-angiogenic activity and markedly reduced the expression of hypoxia-inducible factor 1a. Conclusions: In summary, SB365 inhibit of growth and proliferation through induction of apoptosis in human gliblastoma cell lines. These findings indicate that SB365 might contribute to the growth inhibitory activities in vitro and also might be useful as a good pharmaceutical candidate for use in the treatment of brain tumor.