Development of type II diabetic mellitus animal model with mini-pig

초록

Diabetes has been explosively increased in terms of its incidence and considered one of the serious diseases to overcome for human life. Among the animal models used for the drug efficacy test, mini-pig model is going to rapidly upload because of many similarities with human, particularly concerning the pharmacokinetic of compounds after subcutaneous administration, the structure and function of the gastrointestinal tract, the morphology of the pancreas, and overall metabolic status. Based on these various advantages, we try to develop Type II diabetic mellitus animal model with mini-pig. We set 4 male mini-pigs for the study, 3 animals for the induction of moderate insulin deficient model with Nicotinamide/Streptozotocin treatment and 1 animal for control. For the evaluation of Type II diabetic incidence, we checked blood glucose level, OGTT as well as immunohistochemistry of pancreatic tissue. All of the animals treated showed high glucose and low insulin levels compared to control mini-pig. And also, we got the partially destroyed beta cell population from the tissue of pancreas in the animals treated. Based on these results, we want to report that the animal model developed can be used for efficacy test of candidate diabetic drugs.