Utilization of whole genome treasure for the library construction of industrial enzymes

초록

A huge database resulted from whole genome sequencing has provided a possibility of new information that is likely to extent the scope and thus changes the way of approach for the functional assigning of putative open reading frames annotated by whole genome sequence analyses. These are mainly realized by ease, one-step identification of putative genes using genomics or proteomics tools. A major challenge remained in biotechnology may translate these informations into better ways to screen or select a gene as a representative sequence. Further attempts to mine the related whole genes or partial DNa fragment from whole genome treasure, and then the incorporation of these sequences into a representation template, will result in the use of putative genes that can be translated into functional proteins or allowed the generation of new lineages as a valuable pool. Such screens enable rapid biochemical analysis and easy from the expanded library with related sequences. inforamtion-based PCR amplication of whole genes and reconstitution of functional DNa fragments will provide a platform for expanding the functional spaces or potential enzymes, epecially when used mixed- or metagenome as gene resources