Permianibacter fluminis sp. nov., isolated from a freshwater stream

초록

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated as IMCC34836(T), was isolated from a freshwater stream. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC34836(T) was most closely related to Permianibacter aggregans HW001(T) (of the family Pseudomonadaceae) with 95.6 % sequence similarity and formed a robust clade with P. aggregans HW001(T). The draft genome sequence of strain IMCC34836(T) was 4.4 Mbp in size with 59.1mol% DNA G+C content. Average nucleotide identity and digital DNA-DNA hybridization values between strain IMCC34836(T) and P. aggregans HW001(T) were 71.2 and 22.0%, respectively, indicating that the new strain represents a novel species. The strain contained iso-C-15:0, summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and summed feature 9 (iso-C-17:1 omega 9c and/or C-16:1 10-methyl) as the major fatty acids and harboured phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids as major polar lipids. The isoprenoid quinone detected in the strain was ubiquinone-8. Based on the phylogenetic and phenotypic characteristics, strain IMCC34836(T) is considered to represent a novel species of the genus Permianibacter, for which the name Permianibacter fluminis sp. nov. is proposed. The type strain is IMCC34836(T) (=KACC 21755(T)=NBRC 114416(T)).

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