Angelica gigas Nakai 현탁세포 배양의 동결보존 연구

초록

Cell culture of Angelica gigas Nakai producing decursin and decursinol angelate as secondary metabolites were preserved in liquid nitrogen after pre-freezing in deep freezer(-70C). The development of optimal procedure for cryopreservation was investigated by using cryoprotectant and pre-treatment before cooling. 0.7 M sucrose was found to be the optimum osmotic pre-conditioning culture medium cpmpared to mannitol, sorbitol, and NaCl with the same osmotic pressure. In pre-culture medium, cells in exponential phase, supported the best growth after cryopreservation. Effective cryoprotectant was proved to be a mixture of sucrose, glycerol, DMSO. Higher concentration of cryoprotectant was better for cell viability. The highest relative cell viability established after the development of optimal procedure was 65%.