The nuclear transcription factor NFIB is requiredfor osteogenic and myogenic differentiation of C3H10T1/2 mesenchymal stem cells

초록

The nuclear factor I (NFI) family of transcription factors play important roles in a number of cellular processes including differentiation. In this study, we present evidences that NFIB is required for the differentiation of C3H10T1/2 MSCs. We demonstrate that knockdown of NFIB inhibits osteogenic differentiation of C3H10T1/2 MSCs, which accompanies a significant decrease in expression of key regulators such as Runx2 and Osterix upon osteogenic induction. We also show that osteogenic differentiation can be restored by exogenous expression of NFIB in KD cells. Similarly, we find that depletion of NFIB also hampers myogenic differentiation in both C3H10T1/2 MSCs and C2C12 myoblast cells, which correlates with defects in expression of key myogenic regulators such as MyoD and myogenin. Consistently, impairment of myogenic differentiation in NFIB depleted C2C12 cells can be rescued by exogenous NFIB expression as well as MyoD expression. Taken together, our results suggest that NFIB may play a critical role in maintaining the differentiation potential of MSCs by directly or indirectly regulating lineage-specific transcription factors.