The nuclear factor IB (NFIB) acts as a upstream regulator of Dlx5 dependent osteogenic differentiation in C3H10T1/2 mesenchymal stem cells

초록

The nuclear factor I (NFI) family of transcription factors play important roles in a number of cellular processes including differentiation. In this study, we present evidences that NFIB is required for osteogenic differentiation of C3H10T1/2 MSCs. We demonstrate that knockdown of NFIB results in decreased expressions of key osteogenic transcription factors such as Dlx5, Runx2 and Osterix and thus inhibits osteogenic differentiation in C3H10T1/2 MSCs. Exogenous expression of NFIB restores osteogenic potential in NFIB depleted cells but has no significant effect on differentiation of C3H10T1/2 MSCs. Moreover, we have found that NFIB directly binds to the promoter of dlx5 gene but not to those of runx2 and osterix genes. Furthermore, depletion of NFIB is correlated with decreased level of H3K4me3 in both undifferentiated and differentiated cells. Taken together, our results suggest that NFIB play a critical role in maintaining the differentiation potential of MSCs by directly or indirectly regulating lineage-specific transcription factors.