Genomic Characterization of Strain IMCC26134, a Freshwater Verrucomicrobial Strain Isolated from Lakewater

초록

Strain IMCC26134, a freshwater verrucomicrobial strain, was isolated from Lake Soyang by dilution-to-extinction culturing and formed small colonies on semisolid agar. IMCC26134 is an aerobic microorganism with optimal growth at pH 7.0, at 20°C and salinity ≤ 0.2% NaCl. In phylogenetic analyses, strain IMCC26134 constituted a robust branch within the family Opitutaceae of the phylum Verrucomicrobia. The closest relative of this strain was ‘Candidatus Diplosphaera colotermitum’ with a 16S rRNA gene sequences similarity of 92.6%. Here we describe the features of strain IMCC26134, together with the complete genome sequence information and its annotation. The complete genome was comprised of one circular chromosome, with a length of 4,001,000 bp and a G+C content of 61.3%. The IMCC26134 genome was predicated to contain 3,050 protein-coding genes and 56 genes for RNAs. The majority of the protein coding genes (75.3%) were assigned a putative function. The genome of strain IMCC26134 revealed significant enrichment in genes encoding a wide spectrum of glycoside hydrolases, sulfatases, polysaccharide lyases and carbohydrate esterase, confirming that this organism was well equipped for the hydrolysis of diverse polysaccharides. Strain IMCC26134 genome enhance understanding of the poorly known freshwater Verrucomicrobia phylum.