HCI 프로그램을 이용한 RP-HPLC에서의 펩타이드의 최적 분리 조건

초록

Optimization in reverse phase-high performance liquid chromatography (RP-HPLC) involves the selection of experimental condition for adequate separation and acceptable retention time for each individually samples. But in chemical and pharmaceutical laboratories obtaining a balance between resolution and analysis time is not always easy. An efficient optimization method should be employed during the method development process in order to deal with these optimization problems. Computers have been used as an aid in high performance liquid chromatography method development since the late 1970s.[1] The method developments by software were frequently demonstrated, and the applications using the software have been increasing. Especially HCI program was originally developed by High-Purity Separation Lab., Inha University for the purpose of the optimization of chromatographic separation. Now the scope of HCI program is limited to analytical condition. It can be utilized in normal-phase as well as reversed-phase liquid chromatography for the isocratic and gradient modes. The basic function enables to predict the retention time of samples in a given mobile phase composition, and additionally column efficiency and resolution, elution profile of sample in specific and optimized operating conditions. In this work, the optimization of mobile phase condition for 4 biological-active peptides was determined by HCI program