Nitric Oxide-dependent Suppression of Flavin-containing Monooxygenase Expression in Rat Liver

초록

Liver microsomal FMO activities of LPS-treated rats were decreased significantly. Hepatic content of FMO1 mRNA and the liver microsomal content of FMO1 (the major form in rat liver) in LPS-treated rats were significantly decreased also. However, the expression of inducible NO synthase (iNOS) and plasma nitrate/nitrite (NOx) concentration were increased. In rats cotreated with NOS inhibitors such as aminoguanidine and nitroL-arginine, the ativities and content of FMO1 in liver microsomes were restored. These results suggest that NO is an important mediator involved in the suppression of FMO1 activity in vivo.