Utilization of Pluronic F-68 for hCTLA4Ig Production by Transgenic Rice Cell Cultures in Bioreactor

초록

Transgenic plant cell cultures have been used as a production system for therapeutic proteins. Rice cells were transformed to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). With RAmy3D promoter system, expression of the target protein could be strongly induced by sugar starvation. However, sugar-depleted environment can cause cell death and secretion of proteases into the medium due to cell lysis. In addition, during the mass cultivation and production using bioreactors, various physical factors such as shear stress and bubble burst can damage the cells. In this study, effects of an non-ionic surfactant, Pluronic F-68 (PF-68), as a protective agent in transgenic rice cell cultures were examined to enhance the production. Various concentrations of PF-68 at 1, 3, 5, 7 g/L were added in induction phase. The best hCTLA4Ig production could be obtained with 5 g/L PF-68. It was thought that protective effects against shear stress could enhance hCTLA4Ig production in bioreactor.