Preparative Separation of EGCG from Korean Green Tea by HPLC

초록

Among catechin compounds abundant mainly in green tea, (-)epigallocatechin gallate (EGCG) has anti-oxidative and anticarcinogenic properties. Korean green tea (Bosung, Chonnam) was used as a feed sample. The separation was done by a C18 reversed-phase preparative column (22´250 mm) with mobile phases of 0.1% acetic acid in water, acetonitrile, methanol, and ethyl acetate considered. The injection volume and the flow rate of mobile phase were fixed at 400ml and 16ml/min, respectively. To purify EGCG preparatively, the effluent was collected from the column outlet and concentrated, and analyzed by analytical column (3.9´300 mm). From the experimental results, the optimum mobile phase condition for separating EGCG from Korean green tea was 0.1% acetic acid in water/acetonitrile/methanol/ethyl acetate (80/16/1/3 vol.%). In the experimental condition, 0.6 mg of EGCG was purely obtained.