Casein Kinase 2a Augments Oxaliplatin Resistance in Colorectal Cancer Cells by Increasing ABCE1 Expression

초록

Background/Aim: Antitumor drug resistance is a major hurdle in treating patients with malignant tumors. Casein kinase 2a (CK2a) expression is highly enhanced in oxaliplatin-resistant CRC cells. We investigated whether CK2a expression is associated with oxaliplatin resistance in CRC cells. Materials and Methods: To determine the effect of CK2a on drug resistance in CRC, we assessed the cell viability, adenosine triphosphate-binding cassette (ABC) transporter expression, apoptosis, and sphere formation according to CK2a expression in oxaliplatin-resistant CRC cells. Results: CK2a expression was significantly increased in oxaliplatin resistant CRC cells compared with that in wild-type CRC cells. In addition, the mRNA expression of ABC transporters, including ABCA12, ABCC2, and ABCE1, was significantly enhanced in oxaliplatin-resistant CRC cells, whereas this effect was blocked by the knockdown of CK2a. Furthermore, a cell viability test showed that oxaliplatin resistance was inhibited by decreasing CK2a expression, resulting in the induction of apoptosis and suppression of sphere formation. Conclusion: CK2a regulates cell survival, apoptosis, sphere formation, and drug resistance in oxaliplatin-resistant CRC cells by regulating ABC transporters. Therefore, targeting CK2a in drug-resistant CRC cells may be a novel strategy for treating patients with CRC.

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