Cloning and characterization of glucose transporter isoform from (CSGLUT2) Clonorchis sinensis

초록

The liver fluke (Clonorchis sinensis) infection has been shown, both experimentally and epidemiologically, to cause chronic pathological changes, such as cholangitis, cholecystitis, hepatomegaly, hepatic fibrosis, and cholangiocarcinoma. Several reports have been issued on the cloning, characterization, and functional analysis of Schistosoma mansoni glucose transporters and I have already reported one glucose transporter of Clonorchis sinensis, but NCBI database show several glucose transporter isoforms. In this report, the results for isolation and characterization of other glucose transporter isoform named CsGLUT2,. The full length of CsGLUT2 gene was isolated from total RNA of adult Clonorchis sinensis using reverse transcription polymerase chain reaction. The CsGLUT2 consists of 1491 base pairs that encode a 496 amino acid residue protein. Hydropathy analysis suggested that NCSG possess 10 putative membrane-spanning domains. CsGLUT2 showed 54% amino acid homology to CsGLUT already reported. To clarify the substrate specificity several organic ions, a amino acid and deoxyglucose uptake was determined using Xenopus oocyte expression system. CsGLUT2 showed positive uptake to deoxyglucose only. This updtake was time dependent and sodium ion independent manner. The progressive study on properties of this transporter would give more valuable information to Clonorchis sinensis sugar metabolism.