Hydrolysis of Pulp sludge for Lactic Acid Fermentation using Enzyme System

초록

Enzymatic hydrolysis of cellulose was studied with emphasis on the effect of cellulase loading and pulp sludge concentration on glucose yield. Enzyme loading appeared to have a significant effect on glucose yield. Chemical pretreatment had no effect on enzymatic hydrolysis of pulp sludge. High glucose yield was obtained from enzymatic hydrolysis, especially at sludge concentrations lower than twenty percent. The optimum concentrations of crude cellulase and β-glucosidase were 5 U/mL and 8 U/mL, respectively, considering the amount of enzymes used and glucose produced.