유전자 재조합 E. coli를 이용한 levofloxacin의 광학선택적 생산

초록

Levofloxacin is L-form stereoisomer of ofloxacin. It has better antibacterial activity than D-oflxacin. In this study, levofloxacin was produced enantioselectively by using high density culture of recombinant E. coli containing a foreign esterase gene. Final cell concentration was 89 g/L at the end of fed-batch culture and the cells were used for levofloxacin production after IPTG induction at the optimized condition. For the immobilization of recombinant E. coli, 1.5% sodium alginate showed the best result to maintain enzyme activity and enantioselectivity.