Assessment of genetic diversity and phylogeographic structure of Maackia fauriei and related species (Fabaceae) using microsatellite marker

초록

The Maackia Rupr. & Maxim is an indigenous genus to Eastern Asia and the species are mostly distributed in temperate forest region. M. fauriei is an endemic species restricted in Jeju Island and the following three species are often taxonomically problematic with this species; M. amurensis in E Asia, M. floribunda in S. Japan, and M. hupehensis in China. M. fauriei is similar in standard petal morphology with M. amurensis and in winged legumes with M. floribunda and M. hupehensis. To elucidate genetic structure within and/or among the populations of these species, we isolated microsatellite markers from 39 individuals collected from Mt. Halla, Jeju-do (M. fauriei). Sixteen polymorphic microsatellite markers were developed using selective hybridization protocols. Developed markers was then applied to selected several populations of M. fauriei and related species from China, Korea and Japan. Genetic diversity and phylogeographical structure was analyzed and discussed among these populations. We concluded that the microsatellite markers described here are valuable tools to assess the genetic diversity and phylogeographical structure in M. fauriei and related species.