Characterization of HEK293 and Namalwa cells in culture processes by using simple design of experiment

초록

F2N78, a human somatic hybrid cell line, has been derived from a fusion of human embryonic kidney 293 (HEK293) cells and Namalwa cells. HEK293 has been widely used as a human host for the effective transient expression of recombinant proteins, and Namalwa is a Burkitt’s lymphoma cell line harboring integrated Epstein-Barr Virus (EBV) genome in its chromosome. F2N78 has been adapted to serum-free suspension condition, so that the transfection could be performed in suspension cultures. In this study, we investigated the transient transfection of F2N78 under suspension condition. Higher transfection efficacy and long-lasting cell viability could be obtained compared with other cell lines such as HEK293 and Chinese hamster ovary (CHO-K1) cells usually used for the expression of foreign genes. Furthermore, the growth characteristics and metabolism of F2N78 cell culture were analyzed using different basal media to find an optimal medium. It was found that a chemically defined media named MPC002 provided the best growth conditions. Optimization of MPC002 for the production of recombinant antibody in F2N78 cells is going to be performed for further study.