Rhodoferax lacus sp. nov., isolated from a large freshwater lake

초록

A Gram-staining-negative, aerobic, motile with a single polar flagellum and rod-shaped bacterium as a bacterial host of podovirus P26218, designated IMCC26218(T), was isolated from Lake Soyang, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC26218(T) belonged to the genus Rhodoferax of the family Comamonadaceae and shared 97.7-99.0% sequence similarities with Rhodoferax species. The draft whole genome sequence of strain IMCC26218(T) was ca. 4.9 Mbp in size with the DNA G+C content of 62.3 mol%. Average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values between strain IMCC26218(T) and other Rhodoferax were 74.0-77.3% and 19.5-21.0 %, respectively, showing that the strain represents a new Rhodoferax species. The strain contained summed feature 3 (C-16 : 1 omega 6c and/or C-16 : 1 omega 7c) and C-16 : 0 as the major fatty acids and phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminolipids and two unidentified lipids as major polar lipids. The predominant isoprenoid quinone of the strain was ubiquinone-8 (Q-8). On the basis of the phylogenetic and phenotypic characteristics, strain IMCC26218(T) is considered to represent a novel species of the genus Rhodoferax, for which the name Rhodoferax lacus sp. nov. is proposed. The type strain is IMCC26218(T) (=KACC 18983(T) =NBRC 112709(T)).

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