뇌신경계 허혈 후 발현이 유도되는 유전자의 2차 선별

초록

Hypoxic ischemia produces ischemic excitotoxicity in the brain, which results in massive neuronal death in the targeted areas. Hypothermia was shown to be neuroprotective reducing cerebral infarction in transient focal ischemia. In previous report, we reported a list of genes up-regulated greater than two fold in post ischemic brain. To select physiologically relevant genes among them, we screened the genes, whose induction was suppressed by hypothermic treatment utilizing microarray analysis. Hypothermia (33℃, rectal temperature) was applied for 2h during MCAO and beginning of reperfusion. We investigated the expression levels of previously selected genes at 3 hr, 12 hr, 1 day and 2 days after MCAO and compared them with the result from normothermic treatment. Microarray analysis revealed that intra- and post-ischemic hypothermic treatment suppressed the induction of many genes. After excluding non-specific genes, which were still induced in hypothermic treatment, we selected a group of physiologically relevant genes. It includes genes associated with apoptosis, inflammation, cell proliferation and cell signaling. Functional studies of selected genes are underway.