Titanium dioxide-cellulose hybrid nanocomposite based conductometric glucose biosensor

초록

This paper investigates the feasibility of conductometric glucose biosensor based on glucose oxidase (GOx) immobilized TiO 2-cellulose hybrid nanocomposite. TiO 2 nanoparticles were blended with cellulose solution prepared by dissolving cotton pulp with lithium chloride/N, N-dimethylacetamide solvent to fabricate TiO 2-cellulose hybrid nanocomposite. The enzyme (GOx) was immobilized into this hybrid material by physical adsorption method. The successful immobilization of GOx into TiO 2-cellulose hybrid nanocomposite via covalent bonding between TiO 2 and GOx was confirmed by X-ray photoelectron analysis. The linear response of our propose glucose biosensor is obtained in the range of 1-10mM with correlation coefficient of 0.93. Our study demonstrates TiO 2-cellulose hybrid material as a potential candidate for an inexpensive, flexible and disposable glucose biosensor. © 2012 SPIE.