Plant Cell Cultures in Bioreactors for the Production of mGM-CSF Using Transgenic Nicotiana tabacum Cell Suspensions

초록

Transgenic Nicotiana tabacum cells were cultured for the production of murine granulocyte macrophage-colony stimulating factor (mGM-CSF) in both a stirred tank bioreactor and an airlift bioreactor with draft tube. Cell growth and mGM-CSF production in the airlift bioreactor were found to be better than those achieved in the stirred tank bioreactor. In the airlift bioreactor, 9.0 g/L of cells and 2.2 ng/mL of mGM-CSF were obtained (11.0 g/L and 2.4 ng/mL, respectively in shake flasks). Although the lag period was prolonged and mGM-CSF production was lowered by 33% in the stirred tank bioreactor as compared to the control cultures, the maximum cell density was increased up to 12.0 g/L due to better mixing by agitation at the higher cell density. In conclusion, cell growth and mGM-CSF production using an airlift bioreactor showed patterns similar to those in culture flasks due to the mild culture condition. However, stirred tank bioreactor would be better for high-density culture.