TAA-induced liver damage in KLF10 knockout mice

초록

Aim: To establish an animal model for hepatocellular carcinoma (HCC) with a high occurrence of tumors in short time. Methods: Four groups (mock control, diethylnitrosamine (DEN) for eight weeks, DEN+TAA (thioacetamide) for 4weeks, DEN+TAA for 8weeks) are selected for this study using 14.5 days old C57BL/6 mice. DEN was treated 20mg/kg as starting dose, and 30mg/kg as the second dose, and then 50mg/kg body weight for further 6 doses. For cirrhosis model, additional 4 or 8 doses of TAA 300mg/kg body weight were also given to animals. Results: After six months of first DEN dose, all groups were sacrificed and verify the incidence of HCC using H&E staining and liver damage by ALT and AST level as well as cirrhosis using Sirius red staining and collagen level. Tumors were developed in all animals of the entire group except mock control with high survival ratio. Increased level of the biochemical marker in serum and loss of tissue architecture shows the liver damaged. The hepatocyte death and proliferation is highly activated in all tumor-induced groups. We also found that the collagen level is up-regulated in TAA treated group dose-dependently. Conclusion: These results provide an improved strategy to make an animal model with a high occurrence of tumors and combining with cirrhosis in short time to be used for the researcher who wants to study on liver cancer field