Bioinformatic analysis of molecular evolution of protein kinase C isoforms

초록

In this study, we intended to find out the evolutionary relationship of the PKC isoforms in various species ranging from yeast to human. Here, we extracted the PKC sequences from at least 19 different species from yeast to human and aligned the ePK domain sequences of these proteins to create a phylogenetic tree. Our phylogenetic tree of the PKC isoforms of 19 different species clearly showed the grouping of the PKC isoforms into four branches. Interestingly, we found that the yeast PKCs, namely, Pkc1p from the budding yeast and Pck1 and Pck2 from the fission yeast clustered in the PKCε-PKCη branch of the tree indicating that PKCε and PKCη share the nearest common ancestor with the yeast PKCs. Furthermore, we modeled the three-dimensional structures of the ePK domains from these species and aligned them to see the structural similarities between them.