Extraction and Purification of Eupatilin from Artemisia princeps PAMPAN by Recycling Preparative HPLC

초록

The extracts of the whole herb of Artemisia princeps PAMPAN have been used for the treatment of inflammation, cancer and other disorders. In this experiment, a recycling preparative HPLC was used to extract and separate eupatilin containing in Artemisia princeps PAMPAN, the optimum operating conditions were experimentally determined. Eupatilin was extracted from the leaf and trunk of Artemisia princeps PAMPAN by ethanol. The resulting solution was further partitioned with n hexane, chloroform and ethyl acetate. The solution containing eupatilin was collected by preparative and analytical column, and was then identificated by LC CE MS. In the experiment, the mobile phase was consisted of water/ acetonitrile/TFA=50/50/0.5 (vol. %), UV wavelength was 370 nm. For preparative and analytical chromatography, the injection volume was 20 um and the flow rate was fixed at l.0 ml/min. The measured retention time of eupatilin was approximately 8.6 min. For recycling preparative HPLC, the column used was GSA 310A, the flow rate was fixed at l.5 ml/min and injection volume was 2 ml. This study used 2 times recycling and the purity of eupatilin almost arrived 100 %.