Development of eye exposure characteristic chamber for the identification of environmental disease caused by fine particles-2

초록

Most of studies on adverse effects of air pollution have been focused on respiratory and cardiovascular disease but there has been a relatively few studies on eye diseases following exposure of ambient PMs. PMs are complex mixture of particles that vary in chemical composition or size. In this study, we investigated the influence of collected road dust on cell viability, inflammatory responses, and oxidative stress in human corneal epithelial cells. Collected road dust was classified by aerodynamic diameter and solubility. Total PM2.5 and PM10 decreased cell viability as concentration increased. Pellet fraction of total PM2.5 and PM10 mainly contributed on mitochondrial activity. Although each total PM2.5 and PM10 did not change membrane integrity, its supernatant fraction significantly affected cell membrane integrity. Both total and each fraction induced nitric oxide production and interleukin 8 expression. In addition, total PM2.5 and PM10 increased oxidative stress, even pellet fraction of total PM2.5 and PM10 induced higher oxidative stress. We could not observe the significant difference in cellular responses between total PM2.5 and PM10. However, effects of collected road dust on cellular responses were strongly dependent upon their concentration and solubility.