Separation Characteristics of Oligodeoxynucleotides by High-Performance Membrane Chromatography

초록

Oligodeoxynucleotides(ODNs) were separated by high-performance membrane chromatography (HPMC), a combined system of chromatography and membrane. The separation mechanism involved anion-exchange, and the stationary phases were CIM (Convective Interaction Media) weak anion-exchange DEAE and strong anion-exchange QA disks (16×3 mm). Two types of mobile phase were used, buffer A (20mM Tris-HCI, pH 7.4) and buffer B (buffer A + 1M NaCl). In QA disk, step gradient mode was applied to separate ODNs. On the other hand, in DEAE disk, the reasonable consequences were obtained when applying linear gradient mode.