Genome Analysis of Three Bacterial Strians Isolated from Antarctic Seawater

초록

Here we report genome sequences of 3 bacterial strains isolated from a seawater sample collected nearby the King Sejong Station, Antarctica. Strains IMCC20628, IMCC21224 and IMCC21906 were isolated from seawater by dilution-to-extinction culturing. Genome sequencing was performed by combination of Illumina technology (250 bp paired-end; coverages 241X–396X) and PacBio sequencing (10 kb library; 2 cells/strain; coverages 82X–153X). The genome sequence of strain IMCC20628, affiliated with genus Hoeflea of Alphaproteobacteria, was assembled into 7 contigs, with a total length of 5.13 Mb. Metabolic pathway analysis showed that the IMCC20628 genome had genes for RuMP pathway of formaldehyde utilization and a bacteriorhodopsin. IMCC21224 belonged to genus Puniceibacterium of the marine Roseobacter clade. The IMCC21224 genome was 5.6 Mb long (15 contigs) and encoded genes for carbon monoxide dehydrogenase and SOX (sulfur oxidation) pathway. Strain IMCC21906 was affiliated with genus Spongiibacter of Gammaproteobacteria. The hybrid assembly resulted in a putatively complete genome comprised of 2 contigs (1 chromosome and 1 putative plasmid). The genome was 3.6 Mb long and contained 3504 CDS. Taken together, these genome sequences show the metabolic diversity of bacteria in polar ocean.