담배세포배양을 이용한 재조합 hGM-CSF 생산에서 Pluronic F-68이 미치는 영향

초록

Effect of Pluronic F-68, a nonionic surfactant, on the extracellular production of hGM-CSF in transgenic Nicotiana tabacum cell suspension culture was investigated. The addition of 5 g/L Pluronic F-68 did not affect the cell growth but increased the extracellular production of hGM-CSF by two-fold. This may be due to the enhanced permeability of the cell membrane by the interaction between the Pluronic F-68 and the cell membrane.