Comparison of Growth Characteristics between Periosteum-derived Progenitor Cells and Mesenchymal Stem Cells

초록

Because isolated primary adult stem cells are low in numbers, in vitro expansion is necessary. However, the expansion potential is limited and in vitro aging leads to the loss of multipotency. Therefore, fast cultivation in short time is one of interests in cell therapy. In this study, we found that human periosteum-derived progenitor cells (PDPCs) are more proliferous and glycolytic than primary human mesenchymal stem cells (MSCs). Bone marrow-derived MSCs and umbilical cord blood-derived MSCs were used to compare with PDPCs in terms of growth rate. These cells were counted every 3 days. In addition, we investigated the effects of glucose consumption on the proliferation potential of PDPCs. Glucose consumption and glycogen synthesis were measured by glucose analyser and glucose assay kit. These results show that PDPCs can be used as an alternative source of cell therapy.