Evaluation of biological effect of peptide derived from FBLN1 in stem cell diffentiation

초록

Many extracellular matrix proteins have positive influences on the adhesion, proliferation and differentiation of stem cells into specific cell linages. Here, we investigated the effect of peptide derived from FBLN1 on the ability of human mesenchymal stem cells (hMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p< 0.001) and proliferation of hMSCs (p< 0.05). The ALP and mineralization activities of cells were dramatically increased (p< 0.01) after 9 days and 12 days of FBLN1 treatment, respectively.This, indicated the ability of FBLN1 to induce hMSCs to differentiate into osteoblasts. Furthermore,increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration.