Antarcticibacterium arcticum sp. nov., a bacterium isolated from marine sediment of the Canadian Beaufort Sea

초록

A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-negative, rod-shaped and non-motile bacterial strain, PAMC 28998(T), was isolated from a surface sediment sample collected from the Canadian Beaufort Sea. Strain PAMC 28998(T) grew at 4-37 degrees C (optimum, 25 degrees C), at pH 7.0-9.0 (optimum, pH 7.5) and in the presence of 1.0-10.0% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 28998(T) belongs to the genus Antarcticibacterium showing the highest sequence similarity (96.8%) with Antarcticibacterium flavum JB01H24(T). The average nucleotide identity and genome-to-genome distance values between PAMC 28998(T) and the most closely related species (A. flavum JB01H24(T)) were 74.1 and 18.5%. respectively, indicating that strain PAMC 28998(T) is clearly distinguished from A. flavum. The genomic DNA G+C content calculated from genome sequences was 39.8%. The major fatty acids (>10%) were iso-C-15:0 (19.5%), anteiso-C-15:0 (18.0%), iso-C-16:0 (11.6%) and summed feature 3 (C-1(6:1), omega 6c and/or C-16:1, omega 7c: 11.4%). The major polar lipids were phosphatidylethanolamine. aminoglycolipid, two unidentified aminolipids, three unidentified phospholipids and four unidentified lipids. The major respiratory quinone was MK-6. Based on the phylogenetic, genomic and phenotypic data presented here, strain PAMC 28998(T) is considered to represent a novel species of the genus Antarcticibacterium, for which the name Antarcticibacterium arcticum sp. nov. is proposed with the strain PAMC 28998(T) (=KCCM 43316(T)=JCM 33514(T)).

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