Extraction and Separation of Potential Anti-Cancer Agent in Korean Orange Peel by SFE and RP-HPLC

초록

To extract perillyl alcohol (POH), a potential anti-cancer agent, the peel of Korean orange was extracted by supercritical fluid extraction (SFE) system with CO2 at 50℃, 200 bar and 6 CO2 kg/hr/kg sample. The extracts were partitioned by acetonitrile/hexane (90/10, vol.%). POH was included in the acetonitrile phase. Further, open-tubular chromatography with silica gel (40-63 mm) was used to purify POH from acetonitrile phase. Finally to obtain POH in a pure form, preparative high-performance liquid chromatography was applied. The collection of POH from Korean orange peel was achieved on a laboratory-prepared chromatographic column (300 ´ 3.9 mm) packed with 15mm C18 preparative packings. It was found that the total yield of POH was 1.6 ×10-3 (%, wt.) based on the dry powder of Korean orange peel.