Incomplete N-glycan Processing Leading to Different Consumption Profiles of Carbon, Nitrogen and Phosphorous in Oryza sativa Cultures

초록

In eukaryotic cells, secretory proteins are N-glycosylated in ER and Golgi apparatus. N-glycan engineering is used for the production of recombinant proteins with humanized glycan pattern. N-glycan processing significantly affects physiological properties of host cells. In this study, Oryza sativa of wild type (O.SWT) and N-acetylglucosaminyltransferaseⅠ mutant (O.Sgmt1) were compared in suspension cultures to identify the importance of N-glycan processing for physiological function in plant cells. Consumption profiles of carbon, nitrogen and phosphorous were analysed. As a results, O.SWT predominantly used sucrose as a carbon source, on the other hand, O.Sgmt1 used glucose and fructose derived from sucrose. In the case of nitrogen, uptake profile of ammonium was not different in both type of Oryza stativa. However, O.SWT used up nitrate during exponential phase, while O.Sgmt1 used only half of nitrate during cultivation. These results indicated that N-glycan processing significantly affected physiological function of Oryza sativa leading to different culture profiles. In conclusion, it was found that the culture for glyco-engineered plant cells required medium optimization according to their physiological properties and profiles of nutrient uptake.