Optimization of supplemented whey medium composition for maximum viable cell count of L. crispatus KLB46

초록

Lactobacillus cristatus KLB 46 isolated from Korean woman was grown on supplemented whey medium and medium compositions were optimized for maximum viable cell count. Among the nitrogen sources tested, beef extract yielded the highest viable cell number. When corn steep liquor was applied as an additional nitrogen source, the viable cell number was highest (3.11 × 109 CFU/㎖) in the medium containing 50 g/ℓ corn steep liquor and 10 g/ℓ beef extract. The highest viable cell count (5.00 × 109 CFU/㎖) was obtained from the supplemented whey medium that contains beef extract (10 g/ℓ), corn steep liquor (50 g/ℓ), tween 80 (0.1%, v/v) and trace amounts of sodium acetate (5 g/ℓ), dipotassium phosphate (2 g/ℓ), magnesium sulfate (0.1 g/ℓ), and manganese sulfate (0.05 g/ℓ)