Dopa-conjugated alginate hydrogel glue for rapidhemorrhage control

초록

In this study we prepared tissue-adhesive hemostatic glue and assessed hemostatic effects on hepatic bleeding. animal model. Alginate was used as primary polymer for the fabrication hemostatic glue, oxidized for the introduction of the tissue-adhesive Schiff base forming aldehyde and then encoded with mussel-inspired dopa. In addition, polyallylamine (PAA) was selected for as an intra-structuring polymer which ensures the gel strength and allows instantaneous glue formation on the bleeding spot. Primary glue was quickly formed within 5 to 10 seconds after the mixing oxidized alginate (OA) with PAA. The Dopa-conjugation extent on OA was determined by the tissue adhesion force and the elasticity of the glue (Dopa-OA glue). Especially, elasticity of Dopa-OA glue was significantly enhanced after introduction of dopa to OA. Functional assay of Dopa-OA glue on hepatic bleeding animal model showed much enhanced hemostatic action. Dopa-OA glue is expected to provide novel injectable tissue adhesives for the treatment of hemorrhage caused by clinical procedures or trauma