Media Screening for Perfusion Culture of F2N78 Cells

초록

Mammalian-cell-derived expression system has become the general recombinant protein production platform because of its capacity for post-translational modification and molecular structure assembly similar to human proteins. While characteristics related to production of recombinant protein have been fully understood and Chinese hamster ovary cells are used for the majority of products in field of biopharmaceuticals, significant studies in developing and engineering new cell lines have been progressed. A human hybrid cell line named F2N78 was developed by somatic fusion of human embryonic kidney cells (HEK293) and Namalwa (Burkitt’s Lymphoma) cells. F2N78 cell line inherited advantageous phenotypes such as ease to suspension, high transfection efficacy, EBV genome insertion in chromosome, and human-specific glycosylation. For that reason, this cell line can be used for the production of human therapeutic antibodies or vaccines. In order to enhance the productivity of F2N78 cells, application of perfusion culture was investigated. Before performing perfusion culture, optimal basal and feed media are required for the best growth of F2N78 cells and product formation. The objective of this study is media screening to optimize basal and feed media for perfusion culture of F2N78 cells. Four kinds of basal media (SFM4CHO, Hycell CHO, Ex-cell 293, MPC002-1) and three kinds of feed media (CD Efficient Feed CAGT, Cell boost 5, IS CHO Feed CD XP) were selected for comparison. Through this media screening, MPC002-1 and Cell boost 5 media were selected as basal and feed media, respectively. Using these results, perfusion culture of F2N78 cells will be performed to enhance the protein production.