Flavobacterium rivulicola sp. nov., Isolated from a Freshwater Stream

초록

A Gram-strain-negative, aerobic, yellow-colored, non-motile, and rod-shaped bacterial strain, designated IMCC34852(T), was isolated from a freshwater stream in the Republic of Korea. Cellular growth occurred at 10-37 degrees C, pH 6.0-9.0, and with 0-0.5% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that strain IMCC34852(T) belonged to the genus Flavobacterium and that the strain was most closely related to F. cheonhonense ARSA-15( T) (97.6%), F. buctense T7(T) (96.7%), F. silvisoli RD-2-33( T) (96.1%), and F. paronense KNUS1(T) (96.1%). The whole-genome sequence of strain IMCC34852(T) was 3.2 Mbp in size, with a DNA G + C content 37.3%. The average nucleotide identities (ANI) and digital DNA-DNA hybridization (dDDH) values between strain IMCC34852(T) and its related species were all below 79.8% and 22.7%, respectively, which are significantly lower than the thresholds of 95% for ANI and 70% for DDH for species delineation. The major respiratory quinone of strain IMCC34852(T) was menaquinone-6 (MK-6) and the predominant cellular fatty acids were iso-C-15:0 (32.6%), iso-C-16:0 (11.7%), iso-C-15:1 G (10.3%), and iso-C-14:0 (6.7%). The major polar lipids of the strain were phosphatidylethanolamine, two unidentified aminolipids and six unidentified lipids. Based on these results, it was concluded that strain IMCC34852(T) represents a novel species in the genus Flavobacterium, for which the name Flavobacterium rivulicola sp. nov is proposed. The type strain of the proposed novel species is IMCC34852(T) (= KACC 23133( T) = KCTC 82066( T) = NBRC 114419( T)).

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