Development of a compact mouse retinal imaging system using spectral domain optical coherence tomography (SD-OCT) with a full field stimulation channel for recording fast optoretinograms (ORGs)

초록

While the a-wave of mouse electroretinogram (ERG) occurs within 50 milliseconds after exposure to light, the optoretinogram (ORG) slower than a 20Hz sampling rate could face limitations in observing immediate morphological changes from the retina. In this study, we develop a compact custom-built mouse ORG system based on spectral domain optical coherence tomography (SD-OCT) for 100Hz~1KHz B-scan rates comprised of 100 kHz A-scans. All the optics of the developed ORG system are designed on a 24 x 24 inches optical breadboard to move easily as well as to combine with the ERG system in a dark room. Without using a fundus camera, the OCT system provides en-face images from high-pass filtering and square of the OCT spectral signal for mouse retinal positioning in-vivo before acquiring ORG data. The 490nm LED for light stimulus is generated to make uniform illumination at the mouse retina using the Maxwellian view method. The common path of the OCT scanning light and the visible LED is built with achromatic doublet lens combinations based on optical simulation with Opticstudio® . The developed compact ORG system can not only observe light-evoked responses with 1~10 milliseconds but also be used for the studies of correlations between ORG and ERG in the mouse retina. © 2024 SPIE.

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