한국산 무당개구리의 mtDNA 에서의 Cytochrome b Gene 부분염기서열분석

초록

The polymerase chain reaction(PCR) was used to generate sequences of 716 base pairs(bp) of the mitochondrial cytochrome b protein coding gene from 4 populations of B.orientalis. DNA sequences were compared with 10 individuals of Korean B.orientalis and aligned by comparison with the Xenopus laevis which was analysed full DNA sequences. There are 32bp(4.5%) of substitutions in the B.orientalis with transition and transversion. The ratio of transition and transversion was 2:1, whereas 188bp(26.2%) of base substitutions occured between the outgroup, X.laevis. Among transitions, T and C mismatches are predominated, 76 sites(40.4%), whereas transversions between A and C, A and T are 34 sites (18.6%) and 35 sites (18.9%) respectively. The DNA sequences are 100% homology in Cheju 3 individuals and over 95% homology within species, while 75% homology between outgroup, X.laevis. 3.0%(7 amino acid) of amino acids were substituted from the 238 amino acids translated from the mtDNA 716bp cytochrome b gene within species and differed from 17.6%(42 amino acid) of amino acids with outgroup, X.laevis. Based on Tamura-Nei distance, conservative nucleotide substitutions revealed 0.00-0.05 within species and 0.28 between outgroup.