Effect of Succulents (Dudleya brittonii and Sedeveria pink ruby) Extracts on Poly(I:C)-Induced Inflammation in DF-1 Chicken Fibroblasts

초록

Excessive inflammation in broilers is associated with a decrease in meat productivity, feed intake, and survivability, and consequently has a major impact on the poultry industry. Nowadays, the use of plant-derived substances is increasingly focused on because of their safety and benign properties to prevent and treat inflammation. Therefore, we aimed to evaluate the anti-inflammatory activity of succulents [Dudleya brittonii (DB) and Sedeveria pink ruby (SP)] against polyinosinic:polycytidylic acid [poly(I:C)] induced DF-1 chicken fibroblasts in vitro. To do that, the DF-1 cells were treated with different concentrations of succulent plant extracts and poly(I:C) to investigate dose-dependent effects on cell viability. In addition, the investigation of the cytotoxicity and gene expression of several pro-inflammatory marker genes and mitogen-activated protein kinase (MAPK) genes was also conducted. The results show that when treated with 7 μg/mL and 70 pg/mL, respectively, DB and SP extracts significantly (P<0.05) reduced pro-inflammatory gene expressions such as interleukin 8-like 2 (IL8L2) and interferon regulatory factor 7 (IRF7) that were induced by poly(I:C). Moreover, the DB and SP extracts also strongly reduced the mRNA expression of MAPK genes in the DF-1 cells treated compared with the non-treated group. As a conclusion, the DB and SP extracts showed anti-inflammatory properties against poly(I:C)-induced DF-1 cells, potentially influencing inflammation by altering pro-inflammatory and MAPK gene expression and suggesting succulent plant extracts could be a valuable alternative natural source of inflammation reduction in chickens.

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