Effects of Glucagon-like Peptide-1 on the Insulin Producing Cells Induced from Human Periosteum-derived Progenitor Cells

초록

Treatment of Type 1 diabetes mellitus (T1DM) has limited clinical relevance due to the shortage of pancreas donors. Generation of insulin-producing cells (IPCs) from mesenchymal stem cells (MSCs) is one of promising therapeutic options for future treatment of T1DM. Periosteum-derived progenitor cells (PDPCs) are a kind of MSCs. The cells can be isolated from periosteum using CD9/CD90/CD166 as positive surface markers and they have higher growth potency than the other tissue-derived MSCs. In this study, we investigated the effects of glucagon-like peptide-1 (GLP-1) differentiation of PDPCs into IPCs in vitro. GLP-1, an analog of exendin-4, could stimulate glucose-dependent insulin release from pancreatic islets. It relys on the activation of pancreas duodenum homeobox-1 (PDX-1) in IPCs. Using quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA), production level of insulin can be measured at gene and protein level, respectively. Our results showed the positive effects of the addition of GLP-1. Addition of 10 nM GLP-1 was found to be the optimum for the differentiation of PDPCs into IPCs.