Spatholobus suberectus(SS) Extracts reduced the inflammation of digestive-related disease in animal models

초록

Background: Spatholobus suberectusi is a medicinal plant (Leguminosae) for several disorders such as anemia, rheumatism, abnormal menstruation traditionally used in China. Its stem contains several compounds like eriodictyol, dihydroquercetin, butin, neoisoliquiritigenin, plathymenin, dihydrokaempferol and liquiritigenin. Its potential as a natural extract for medicine, functional food and cosmetic still remains. In the present study we have investigated its role on anti-inflammation in digestive-related animal disease models. Methods: We divided the animals group as following: (1) vehicle control, (2) disease control, (3) 10-2 SS extracts, (4) 10-1 SS extracts, (5) positive drug control (gastritis model only). To make gastritis model, indomethacin was given at 30 minutes after SS extracts administration. 25 mg/kg indomethacin was given to all rats (n=10) by oral gavage in 3% sodium bicarbonate. Seven hours after, the entire rats were sacrificed and confirmed gastrointestinal erosions and ulcers, and then verified further using histological analysis. To make colitis model, 6-8 weeks’ mice (n=10) was given 2% DSS in distilled water ad-libitum during 7days following replaced regular drinking water for 1day. Mice were daily checked the body weight, bleeding and diarrhea for DAI index. At the autopsy, the rectum was photographed, measured the length and then further progressed the Swiss roll for histological analysis. Results: Spatholobus suberectusi extracts decreased the score of gastric damage in gastritis disease model (P<0.05) as well as reduced the colon length shortening in colitis animal model (P<0.05) as a dose dependent manner. It is confirmed on histological index data with H&E staining with gut and colon mucosal damage as well as DAI index in colitis model. And also, the entire tissue section of each group treated the extracts showed decreasing the expression of inflammation markers such as COX2, TNF-? and iNOS.