배양환경에 따른 Thraustochytrium aureum(ATCC34304)의 DHA(docosahexaenoic acid)생산과 성장특성

초록

Shaking cultivation was carried out in 250 mL Elenmeyer flasks containing 60 mL of culture solution for 7 days. Artificial seawater was used as a basal culture medium. The cultivation was performed under the conditions that the temperature ranged from 4 ℃ to 32 ℃, the agitation speed 50 rpm to 200 rpm, and the sugar concentration 5 g/L to 35 g/L. The medium condition was cultured that sugar and nitrogen ratio 1.25 to 10. The biomass, the lipid content in biomass, and the DHA content of lipid were investigated according to the cultivation conditions. The lipid in biomass was distributed between 10 and 34 %, the DHA in lipid 34% to 43% of the lipid, and the biomass concentration 0.425 g/L to 4.50 g/L.