Cultivation and Genome Characteristics of Strain IMCC19250 Belonging to the LD28 Clade, Isolated from Soyang Lake

초록

The LD28 clade of the family Methylophilaceae is a betaproteobacterial lineage widely found in freshwater environments. Although many environmental sequences have been found to be affiliated with the LD28 clade, no cultured isolate has been reported so far. Here we report cultivation and genome sequencing of strain IMCC19250 of the LD28 clade, isolated from Soyang Lake. Strain IMCCI9250 showed ;:;95% 16S rRNA gene sequence similarity to HTCC2181, an isolate of marine methylotrophic OM43 clade. Addition of methanol was revealed to markedly promote the growth of IMCC19250, suggesting a methylotrophy as reported for HTCC2181. The draft genome sequence of IMCC19250was deterrnllled by Illumina sequencing (250-bp paired-end), resulting in 5 contigs. Through combinatorial PCR-based contig ordering and gap filling, complete genome sequence was obtained The IMCC19250 genome was 1.31-Mbp long with a G+C content of 36.6%. Genes for methylotrophy were predicted from the genome, including a gene for methanol dehydrogenase, as expected from thε utilization of methanol. A putative xanthorhodopsin gene was also predicted, suggesting also a photoheterotrophic metabolism of the new strain.