Effects of Pluronic F-68 and polyethylene glycol on the production of mGM-CSF by transgenic <i>Nicotiana tabacum</i> suspension cell culture

초록

The feasibility of using Pluronic F-68 and polyethylene glycol (PEG) as a medium additive in cell suspension cultures of transgenic <i>Nicotiana tabacum</i> was investigated in a 5-L stirred tank bioreactor. The addition of optimum amounts of Pluronic F-68, PEG 8,000, and PEG 20,000 in the culture media promoted cell growth and the production of recombinant murine granulocyte-macrophage colony stimulating factor (mGM-CSF). Specific growth rates were enhanced by 74.6, 39.6, and 47.4% when Pluronic F-68, PEG 8,000, and PEG 20,000 were added respectively. Maximum cell density was also increased by up to 21.4% when 1 g/L of Pluronic F-68 was added as supplement. In terms of the production of mGM-CSF, PEG 8,000 showed the best stimulatory effect. The presence of 2 g/L of PEG 8,000 in the culture medium increased the concentration of mGM-CSF 4-fold from 1.64 to 6.60 ng/mL.