Separation of Eupatilin from Artemisia princeps PAMPAN by Recycling HPLC

초록

In this experiment, the extraction and separation of eupatilin, contained in Artemisia princeps PAMPAN was carried out using a preparative chromatography, and the optimum operating conditions were experimentally determined. Eupatilin was extracted from the leaf and trunk of Artemisia princeps PAMPAN by ethanol. The resulting solution was further partitioned with n hexane, chloroform and ethyl acetate. The mobile phase composition and the injection volume were water/ acetonitrile/TFA=50/50/0.5 (vol.%), respectively. The measured retention time of eupatilin was approximately 8.6 min. The solution which contained eupatilin was collected and identificated by LC CE MS.