Effects of Surface Condition on the Differentiation of Periosteum-derived Progenitor Cells into Insulin-producing Cells

초록

Type 1 diabetes mellitus (T1DM) has been known as a disease caused by T cell-mediated autoimmune destruction of pancreatic cells. Generation of glucose-responsive insulin-producing cells (IPCs) from human mesenchymal stem cells (MSCs) has immense potential for the treatment of T1DM due to the shortage of pancreas donors. Periosteum-derived progenitor cells (PDPCs) are a kind of MSCs and obtained by sorting from the cells in periosteum. They have higher growth potency than the other tissue-derived MSCs. In this study, we compared two different types of surface conditions for in vitro differentiation of PDPCs into IPCs. Poly-D-lysine or collagen type 1 was coated on the culture plates for adhesion cell cultures of PDPCs. For comparison, non-treated culture plates were used for suspension cell cultures. Induced IPCs was confirmed by using immunofluorescence. Enzyme-linked immunosorbent assay (ELISA) was used to measure insulin. By using real-time quantitative polymerase chain reaction (RT-qPCR), expression levels of insulin genes were detected. In conclusion, IPCs derived from PDPCs in suspension condition on non-treated plates showed superior expression of insulin gene than in adhesion condition on coating plates. However regardless of surface conditions, the glucose-stimulated insulin secretion of IPCs was found to be similar.