Sub/Supercritical Fluid Extraction of Aglycone Isoflavones in Korean Soybean

초록

Isoflavones are phytochemicals found in a variety of plants including soybean. Isoflavones, such as glycosides daidzin, glycitin, and genistin, are quite abundant in soybeans. Aglycones such as daidzein, glycitein and genistein, derived from these glycosides, are frequently examined in their chemical structures. The natural presence in the soy of isoflavones, mainly genistein and daidzein are the two major isoflavonoids in soybean, and were thought to play important roles in cancer prevention. In this work, the extraction and separation of isoflavones from Korean soybean were performed by supersonification and sub/supercritical water extraction. And the various experimental variables were the temperature and time in supersonification the temperature and pressure of supercritical water. From the experimental results of the variation of solvent extraction by change in composition, the increase in extraction of a specific compound by supersonic energy and sub/supercritical fluid, the supersonification was the most desirable extraction method in extracting aglycone isoflavones, daidzein and genistein, from Korean soybean. The experimental conditions of temperature and time were 25℃ and 60 min, respectively.